Physical Map Publication
Palaeohexaploid Ancestry for Caryophyllales Inferred from Extensive Gene-Based Physical and Genetic Mapping of the Sugar Beet Genome (Beta vulgaris)
Dohm JC, Lange C, Holtgräwe D, Rosleff Sörensen T, Borchardt D, Schulz B, Lehrach H, Weisshaar B, Himmelbauer H. Plant J. 2012 May;70(3):528-40.
Sugar beet (Beta vulgaris) is an important crop plant accounting for 30% of the world's sugar production annually. The genus Beta is a distant relative of currently sequenced taxa within the core eudicotyledons, and genomic characterization of sugar beet is essential in order to make its genome accessible to molecular dissection. Here, we present comprehensive genomic information in genetic and physical maps covering all nine chromosomes. Based on this information we identified... Read more
- Haplotype divergence in Beta vulgaris and microsynteny with sequenced plant genomes
Dohm JC, Lange C, Reinhardt R, Himmelbauer H. Plant J. 2009 Jan;57(1):14-26
We characterized two overlapping sugar beet (Beta vulgaris) bacterial artificial chromosome (BAC) clones representing different haplotypes. A total of 254 kbp of the genomic sequence was determined of which the two BACs share 92 kbp. Eleven of 15 genes discovered in the sequenced interval locate to the overlap region. The haplotypes differ in exons by 1% (nucleotide level) and in non-coding regions by... Read more
- Construction and characterization of a sugar beet (Beta vulgaris) fosmid library
Lange C, Holtgräwe D, Schulz B, Weisshaar B, Himmelbauer H. Genome. 2008 Nov;51(11):948-51
A sugar beet (Beta vulgaris) fosmid library from the doubled haploid accession KWS2320 encompassing 115,200 independent clones was constructed and characterized. The average insert size of the fosmid library was determined by pulsed field gel electrophoresis to be 39 kbp on average, thus representing 5.9-fold coverage of the sugar beet genome (758 Mbp). PCR screening of plate pools with primer pairs... Read more
- Analysis of DNA polymorphisms in sugar beet (Beta vulgaris L.) and development of an SNP-based map of expressed genes.
Schneider K, Kulosa D, Soerensen TR, Möhring S, Heine M, Durstewitz G, Polley A, Weber E, Jamsari, Lein J, Hohmann U, Tahiro E, Weisshaar B, Schulz B, Koch G, Jung C, Ganal M. Theor Appl Genet. 2007 Sep;115(5):601-15
A panel of 13 sugar beet lines and one genotype each of the Beta vulgaris cultivars red beet and Swiss chard, and B. vulgaris ssp. maritima were used to identify polymorphisms in alignments of genomic DNA sequences derived from 315 EST- and 43 non-coding RFLP-derived loci. In sugar beet lines, loci of expressed genes showed an average SNP frequency of... Read more